Angiopoietin 2 antibody (N-Term)

Catalog No. ABIN95113

Supplier Product No.: 100-401-402

Product Details anti-Angiopoietin 2 Antibody

Target: Angiopoietin 2 (ANGPT2)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Multiplex Assay (MA)

Supplier Product No.: 100-401-402

Supplier: Rockland

Purpose: Angiopoietin 2 Antibody

Cross-Reactivity (Details): Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins.

Characteristics: Synonyms: rabbit anti-Angiopoietin 2 Antibody, rabbit anti-ANG2 antibody, AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Angiopoietin 2 Antiserum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200-1:500

Application Note: Angiopoietin 2 Antibody is tested in WB, ICC, and IHC. Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000 - 1:25,000

Other: ICC: 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Angiopoietin 2 antibody (ABIN95113)

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Davis, Aldrich, Jones, Acheson, Compton, Jain, Ryan, Bruno, Radziejewski, Maisonpierre, Yancopoulos: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, (1997) (PubMed).

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angpt2 (ANGPT2 Products)

Background: Background: Anti-Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) which has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation.  Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. This antibody is suitable for cardiovascular research.

Gene ID: 11601

NCBI Accession: NP_031452

UniProt: O35608

Pathways: RTK Signaling