Angiopoietin 1 antibody (AA 15-45)

Catalog No. ABIN95109

Supplier Product No.: 100-401-403

Product Details anti-Angiopoietin 1 Antibody

Target: Angiopoietin 1 (ANGPT1)

Binding Specificity: AA 15-45

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Supplier Product No.: 100-401-403

Supplier: Rockland

Purpose: Angiopoietin 1 Antibody

Cross-Reactivity (Details): Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1.

Characteristics: Synonyms: rabbit anti-Angiopoietin 1 Antibody, rabbit anti-ANG1 antibody, AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody

Sterility: Sterile filtered

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200-1:800

Application Note: Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal.  Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights.  In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

Western Blot Dilution: 1:500-1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Angiopoietin 1 antibody (ABIN95109)

Egginton, Hussain, Hall-Jones, Chaudhry, Syeda, Glen: "Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible." in: Acta physiologica (Oxford, England), Vol. 218, Issue 3, pp. 153-166, (2017) (PubMed).

Dallabrida, Ismail, Pravda, Parodi, Dickie, Durand, Lai, Cassiola, Rogers, Rupnick: "Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 3010-23, (2008) (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: Angpt1 (ANGPT1 Products)

Background: Background: Anti-Antiopoietin-1 Antibody, which recognizes Angiopoietin-1 (Ang-1), has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver. Anti-Antiopoietin-1 Antiserum antibody is suitable for cardiovascular research.

Gene ID: 11600, 46048213

UniProt: O08538

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process