ADIPOQ antibody

Catalog No. ABIN950284

Product Details anti-ADIPOQ Antibody

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ADIPOQ antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Mouse Adiponectin. It is crossreactive to Human Adiponectin protein in Western blotting.

Purification: Protein A/G Affinity Chromatography

Sterility: 0.2 μm filtered

Immunogen: Purified Mouse Recombinant Adiponectin.

Clone: 7J61

Isotype: IgG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL

Buffer: 0.2 μm filtered solution in PBS

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months.

Expiry Date: 6 months

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin (ADIPOQ Products)

Background: Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an antiinflammatory agent, but it exerts proinflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin13/ T-Cadherin, as well as with several growth factors. Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C terminal C1q like globular domain which can be cleaved by a leukocytederived elastase. Mature mouse adiponectin shares 83 % and 91 % amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities.Synonyms: 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADIPOQ, APM1, Adipocyte, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, C1q and collagen domain-containing protein, GBP28, Gelatin-binding protein

Gene ID: 11450

NCBI Accession: NP_033735

UniProt: Q60994