Amylase, alpha antibody

Catalog No. ABIN95004

Supplier Product No.: 200-401-045

Product Details anti-Amylase, alpha Antibody

Target: Amylase, alpha (AMY)

Reactivity: Bacillus amyloliquefaciens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase, alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-401-045

Supplier: Rockland

Purpose: Alpha Amylase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].

Characteristics: Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Purification: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 μg of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Alpha Amylase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Alpha Amylase Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Amylase, alpha

Target: Amylase, alpha (AMY)

Alternative Name: Alpha-amylase (AMY Products)

Background: Background: Alpha Amylase is a protein enzyme that preforms endohydrolysis of alpha bonds in large alpha linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Alpha Amylase applications include ethanol production and reduction of starch or glycogen molecules. Anti-Alpha Amylase Antibody is useful for researchers interested in Metabolism, Cancer, Cell Signaling, and Signal Transduction research.

Gene ID: 7849308

NCBI Accession: WP_013352208

UniProt: P00692