Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN94946

Supplier Product No.: 100-401-142

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-401-142

Supplier: Rockland

Purpose: ALKALINE PHOSPHATASE Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Human Intestine].

Characteristics: Synonyms: rabbit anti-Alkaline phosphatase antibody, Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Alkaline Phosphatase [Human Intestine]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Alkaline Phosphatase has been tested by western blot and is suitable to be assayed against 1.0 μg of Alkaline Phosphatase [Human Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Abstract: ALPI Products

Background: Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.

Gene ID: 248

NCBI Accession: NP_001622

UniProt: P09923