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Alkaline Phosphatase antibody

Rockland 100-4134 ALP Reactivity: E. coli WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN94942
Supplier Product No.: 100-4134
  • Target See all Alkaline Phosphatase (ALP) Antibodies
    Alkaline Phosphatase (ALP)
    Reactivity
    • 50
    • 16
    • 12
    • 11
    • 11
    • 1
    • 1
    E. coli
    Host
    • 50
    • 17
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 45
    • 26
    Polyclonal
    Conjugate
    • 40
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Alkaline Phosphatase antibody is un-conjugated
    Application
    • 42
    • 25
    • 19
    • 18
    • 11
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    100-4134
    Supplier
    Rockland
    Purpose
    Alkaline Phosphatase Antibody
    Characteristics
    Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
    Purification
    Anti-Alkaline Phosphatase (E.Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Alkaline Phosphatase [E.coli]

    Immunogen Type: Native Protein

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    Discover our top product ALP Primary Antibody
  • Application Notes

    Application Note: Anti-Alkaline Phosphatase (E. Coli) Antibody has been tested against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:8,000 - 1:40,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    75 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Epstein, Kiechle, Artiss, Zak: "The clinical use of alkaline phosphatase enzymes." in: Clinics in laboratory medicine, Vol. 6, Issue 3, pp. 491-505, (1986) (PubMed).

    Lange, Millan, Stigbrand, Vessella, Ruoslahti, Fishman: "Placental alkaline phosphatase as a tumor marker for seminoma." in: Cancer research, Vol. 42, Issue 8, pp. 3244-7, (1982) (PubMed).

  • Target
    Alkaline Phosphatase (ALP)
    Alternative Name
    Alkaline Phosphatase (ALP Products)
    Background
    Background: Alkaline Phosphatase is an enzyme that is key in the hydrolysis of many different types of molecules, resulting in the removal of phosphate groups from said molecule. Most active in basic environments and a common serum analyte, alkaline phosphatase and its isoenzymes are good indicators of bone, liver, and testicular diseases/cancers in immunoassays and diagnostics. Anti-Alkaline Phosphatase (E. Coli) Antibody is ideal for investigators in Cancer, Immunology, and Molecular Biology.
    Gene ID
    945041
    NCBI Accession
    NP_414917
    UniProt
    P00634
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