Alkaline Phosphatase antibody
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- Target See all Alkaline Phosphatase (ALP) Antibodies
- Alkaline Phosphatase (ALP)
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Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Alkaline Phosphatase antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 100-4134
- Supplier
- Rockland
- Purpose
- Alkaline Phosphatase Antibody
- Characteristics
- Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
- Purification
- Anti-Alkaline Phosphatase (E.Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Alkaline Phosphatase [E.coli]
Immunogen Type: Native Protein
- Top Product
- Discover our top product ALP Primary Antibody
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- Application Notes
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Application Note: Anti-Alkaline Phosphatase (E. Coli) Antibody has been tested against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:8,000 - 1:40,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 75 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Alkaline Phosphatase (ALP)
- Alternative Name
- Alkaline Phosphatase (ALP Products)
- Background
- Background: Alkaline Phosphatase is an enzyme that is key in the hydrolysis of many different types of molecules, resulting in the removal of phosphate groups from said molecule. Most active in basic environments and a common serum analyte, alkaline phosphatase and its isoenzymes are good indicators of bone, liver, and testicular diseases/cancers in immunoassays and diagnostics. Anti-Alkaline Phosphatase (E. Coli) Antibody is ideal for investigators in Cancer, Immunology, and Molecular Biology.
- Gene ID
- 945041
- NCBI Accession
- NP_414917
- UniProt
- P00634
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