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Calf Intestinal Alkaline Phosphatase (CIAP) antibody

Rockland 200-6135-0100 CIAP Reactivity: Cow WB, ELISA Host: Sheep Polyclonal unconjugated
Rockland
Catalog No. ABIN94939
Supplier Product No.: 200-6135-0100
  • Target See all Calf Intestinal Alkaline Phosphatase (CIAP) products
    Calf Intestinal Alkaline Phosphatase (CIAP)
    Reactivity
    Cow
    Host
    • 4
    • 2
    • 1
    Sheep
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 3
    • 3
    • 1
    Un-conjugated
    Application
    • 7
    • 6
    • 4
    • 2
    Western Blotting (WB), ELISA
    Supplier Product No.
    200-6135-0100
    Supplier
    Rockland
    Purpose
    Alkaline Phosphatase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
    Characteristics
    Synonyms: sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
    Purification
    Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Alkaline Phosphatase [Calf Intestine]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: ALKALINE PHOSPHATASE antibody has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:4,000 - 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Calf Intestinal Alkaline Phosphatase (CIAP)
    Alternative Name
    Calf Intenstinal Alkaline Phosphatase (CIAP Products)
    Background
    Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
    Gene ID
    280993
    UniProt
    P19111
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