Calf Intestinal Alkaline Phosphatase (CIAP) antibody
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- Target See all Calf Intestinal Alkaline Phosphatase (CIAP) products
- Calf Intestinal Alkaline Phosphatase (CIAP)
- Reactivity
- Cow
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 100-6135
- Supplier
- Rockland
- Purpose
- Alkaline Phosphatase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alkaline Phosphatase [Calf intestine].
- Characteristics
- Synonyms: sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Alkaline Phosphatase [Calf intestine]
Immunogen Type: Native Protein
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- Application Notes
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Application Note: Anti-Alkaline Phosphatase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 33.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Calf Intestinal Alkaline Phosphatase (CIAP)
- Alternative Name
- Calf Intenstinal Alkaline Phosphatase (CIAP Products)
- Background
- Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
- Gene ID
- 280993
- UniProt
- P19111
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