Alkaline Phosphatase antibody

Catalog No. ABIN94935

Product Details anti-Alkaline Phosphatase Antibody

Target: Alkaline Phosphatase (ALP)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Endotoxin Level: Low Endotoxin : No

Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: ELISA Dilution: 1:20.000 - 1:100.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-Alkaline Phosphatase antibody (ABIN94935)

Jones, Brindley, Elder, Myers, Rajala, Dekaney, McNamee, Frey, Shroyer, Dempsey: "Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 3, pp. 533-554, (2019) (PubMed).

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase (ALP Products)

Background: Synonyms: Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Gene ID: 280993

UniProt: P19111