ALD2 antibody

Catalog No. ABIN94896

Supplier Product No.: 200-4144-0100

Product Details

Target: ALD2

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Supplier Product No.: 200-4144-0100

Supplier: Rockland

Purpose: Aldehyde Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].

Characteristics: Synonyms: rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD

Purification: Anti-Aldehyde Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Aldehyde Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aldehyde Dehydrogenase has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: ALD2

Background: Background: The enzyme encoded by this gene belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. The ALDH2 gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix, in contrast the ALDH1 gene codes for the cytosolic isoform. Anti-ALDEHYDE DEHYDROGENASE is ideal for researched interested in metabolism research.

Gene ID: 855206

NCBI Accession: NP_013893

UniProt: P47771