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Aldehyde Dehydrogenase antibody

ALDH Reactivity: Saccharomyces cerevisiae WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN94896
  • Target See all Aldehyde Dehydrogenase (ALDH) Antibodies
    Aldehyde Dehydrogenase (ALDH)
    Reactivity
    Saccharomyces cerevisiae
    Host
    • 13
    Rabbit
    Clonality
    • 13
    Polyclonal
    Conjugate
    • 7
    • 5
    • 1
    This Aldehyde Dehydrogenase antibody is un-conjugated
    Application
    • 12
    • 11
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Aldehyde Dehydrogenase from other tissues and species may occur but have not been specifically determined.
    Purity
    Anti-ALDEHYDE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Aldehyde Dehydrogenase [Yeast]
    Immunogen Type: Native Protein
    Isotype
    IgG
    Top Product
    Discover our top product ALDH Primary Antibody
  • Application Notes
    Anti-ALDEHYDE DEHYDROGENASE should be optimized by tthe end user for specific conditions for reactivity.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:2.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Aldehyde Dehydrogenase (ALDH)
    Alternative Name
    Aldehyde Dehydrogenase (ALDH Products)
    Synonyms
    146084_at antibody, ACDH antibody, ALDH antibody, CG 3752 antibody, CG3752 antibody, Dmel\\CG3752 antibody, GB12741 antibody, Tb06.26G9.1140 antibody, Aldehyde dehydrogenase antibody, ALdehyde deHydrogenase antibody, aldehyde dehydrogenase, mitochondrial antibody, aldehyde dehydrogenase antibody, betaine-aldehyde dehydrogenase antibody, ran-specific GTPase-activating protein antibody, Aldh antibody, alh-6 antibody, alh-12 antibody, LOC550687 antibody, aldH antibody, Tb927.6.4210 antibody, HQ_RS05000 antibody, aldA-2 antibody, ALDH antibody, LOC618976 antibody
    Background
    The enzyme encoded by this gene belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. The ALDH2 gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix, in contrast the ALDH1 gene codes for the cytosolic isoform. Anti-ALDEHYDE DEHYDROGENASE is ideal for investigatorsSynonyms: Aldehyde dehydrogenase [NAD(P)+] 1 ALD2, ALD5
    Gene ID
    855206
    UniProt
    P47771
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