Alcohol Dehydrogenase (ADH) antibody
-
- Target See all Alcohol Dehydrogenase (ADH) Antibodies
- Alcohol Dehydrogenase (ADH)
-
Reactivity
- Saccharomyces cerevisiae
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
- Purity
- Anti-ALCOHOL DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].
- Endotoxin Level
- Low Endotoxin : No
- Immunogen
-
Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein - Isotype
- IgG
-
-
- Application Notes
-
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:2.000 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Handling Advice
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Expiry Date
- Expiration date is one (1) year from date of opening.
-
- Target
- Alcohol Dehydrogenase (ADH)
- Alternative Name
- Alcohol Dehydrogenase (ADH Products)
- Background
- Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1 Alcohol dehydrogenase I YADH-1
- Gene ID
- 2538902, 12643994
- UniProt
- P00330
-