ADH1 antibody
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- Target See all ADH1 Antibodies
- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
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Reactivity
- Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ADH1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Supplier Product No.
- 100-4143
- Supplier
- Rockland
- Purpose
- Alcohol Dehydrogenase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alcohol Dehydrogenase [Yeast].
- Characteristics
- Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.
Immunogen Type: Native Protein
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- Discover our top product ADH1 Primary Antibody
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- Application Notes
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Application Note: Anti-Alcohol Dehydrogenase Antibody is suitable for use in ELISA and western blot.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 90 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
- Alternative Name
- ADH1 (ADH1 Products)
- Background
- Background: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.
- Gene ID
- 2538902, 12643994
- UniProt
- P00330
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