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ADH1 antibody

Rockland 100-4143 ADH1 Reactivity: Saccharomyces cerevisiae WB, IP, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN94889
Supplier Product No.: 100-4143
  • Target See all ADH1 Antibodies
    ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
    Reactivity
    • 8
    • 2
    • 2
    Saccharomyces cerevisiae
    Host
    • 11
    • 1
    Rabbit
    Clonality
    • 11
    • 1
    Polyclonal
    Conjugate
    • 7
    • 3
    • 2
    This ADH1 antibody is un-conjugated
    Application
    • 12
    • 10
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Supplier Product No.
    100-4143
    Supplier
    Rockland
    Purpose
    Alcohol Dehydrogenase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alcohol Dehydrogenase [Yeast].
    Characteristics
    Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.

    Immunogen Type: Native Protein

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    Discover our top product ADH1 Primary Antibody
  • Application Notes

    Application Note: Anti-Alcohol Dehydrogenase Antibody is suitable for use in ELISA and western blot.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    90 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
    Alternative Name
    ADH1 (ADH1 Products)
    Background
    Background: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone.  Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer.  This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization.  Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.
    Gene ID
    2538902, 12643994
    UniProt
    P00330
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