ATP1A1 antibody

Catalog No. ABIN94710

Supplier Product No.: 200-9155

Product Details anti-ATP1A1 Antibody

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Dog

Host: Chicken

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-9155

Supplier: Rockland

Purpose: ATPase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].

Characteristics: Synonyms: chicken anti-ATPase Antibody, Sodium/potassium-transporting ATPase

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-ATPase Na+ K+ has been assayed against 1.0 μg of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1 (ATP1A1 Products)

Background: Background: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Gene ID: 403992

NCBI Accession: NP_001003306

UniProt: P50997

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process