This product has been assayed by western blot and was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms. A dilution of 1:100 to 1:500 is suggested for immunoblotting followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] Rabbit) (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Target
ATPase (DNAH8)
(Dynein, Axonemal, Heavy Chain 8 (DNAH8))