NAM-DH antibody (Biotin)

Catalog No. ABIN94628

Supplier Product No.: 200-106-228-0100

Product Details

Target: NAM-DH

Reactivity: Pseudomonas

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Supplier Product No.: 200-106-228-0100

Supplier: Rockland

Purpose: N-Acylmannosamine-1-Dehydrogenase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase [Recombinant from Pseudomonas].

Characteristics: Synonyms: goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody biotin Conjugation, Biotin Conjugated goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH

Purification: Anti-N-Acylmannosamine-1-Dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-N-Acylmannosamine-1-Dehydrogenase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: NAM-DH

Background: Background: Anti-N-Acylmannosamine-1-Dehydrogenase recognizes the protein N-Acylmannosamine 1-Dehydrogenase, a member of the oxidoreductase family that act on CH-OH donors and NAD+ or NADP+ as acceptors. N-acyl-D-mannosamine 1-dehydrogenase catalyzes the reaction in which N-acyl-D-mannosamine and NAD+ are converted to N-acyl-D-mannosaminolactone, NADH, and H+.

Gene ID: 7614553

UniProt: P22441