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Kinesin antibody (AA 331-906)

Reactivity: Human, Mouse, Pig WB, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN94498
  • Target See all Kinesin products
    Kinesin
    Binding Specificity
    • 16
    • 2
    • 1
    • 1
    • 1
    AA 331-906
    Reactivity
    • 21
    • 19
    • 4
    • 3
    • 2
    Human, Mouse, Pig
    Host
    • 19
    • 6
    Rabbit
    Clonality
    • 19
    • 6
    Polyclonal
    Conjugate
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Kinesin antibody is un-conjugated
    Application
    • 14
    • 13
    • 13
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunocytochemistry (ICC)
    Specificity
    The polyclonal antibody detects total level of endogenous kinesin protein (intracellular antigen).
    Cross-Reactivity (Details)
    Human, Mouse, Porcine, Other not tested
    Purification
    Purified by ligand affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).

  • Target
    Kinesin
    Abstract
    Kinesin Products
    Synonyms
    kinesin antibody, putative kinesin antibody, Tc00.1047053510323.120 antibody, Tc00.1047053508153.200 antibody, LmjF29.2390 antibody, LINJ_29_2500 antibody, LBRM_29_2360 antibody
    Background
    Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KIN
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