TNC antibody

Catalog No. ABIN94480

Product Details anti-TNC Antibody

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody T2H5 recognizes tenascin C, a large hexameric extracellular matrix glycoprotein.

Cross-Reactivity (Details): Other not tested, Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Protein preparation from a homogenate of a human mammary tumour specimen.

Clone: T2H5

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNC antibody (ABIN94480)

Gulubova: "Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis." in: Pathology, research and practice, Vol. 198, Issue 12, pp. 803-12, (2003) (PubMed).

Faustino, van Garderen, Schalken, Nederbragt: "Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues." in: Journal of comparative pathology, Vol. 126, Issue 1, pp. 1-8, (2002) (PubMed).

Ioachim, Charchanti, Briasoulis, Karavasilis, Tsanou, Arvanitis, Agnantis, Pavlidis et al.: "Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and ..." in: European journal of cancer (Oxford, England : 1990), Vol. 38, Issue 18, pp. 2362-70, (2002) (PubMed).

Tökés, Hortoványi, Csordás, Kulka, Mózes, Hatalyák, Kádár: "Immunohistochemical localisation of tenascin in invasive ductal carcinoma of the breast." in: Anticancer research, Vol. 19, Issue 1A, pp. 175-9, (1999) (PubMed).

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin (TNC Products)

Synonyms: tn antibody, 150-225 antibody, GMEM antibody, GP antibody, HXB antibody, JI antibody, TN antibody, TN-C antibody, AI528729 antibody, C130033P17Rik antibody, Hxb antibody, Ten antibody, cytotactin antibody, tenascin-C antibody, P230 antibody, tenc antibody, wu:fk04d02 antibody, tenascin C L homeolog antibody, tenascin C antibody, tnc.L antibody, TNC antibody, MCYG_00667 antibody, tnc antibody, Tnc antibody

Background: Tenascin C,Tenascin C is an approximately 250 kDa extracellular matrix glycoprotein with important roles in the nervous system, as it promotes correct migration of growing axons during development and during neuronal regeneration. It is also involved in synaptic plasticity. Ligands of tenascin C are integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3, and alpha-V/beta-6. Similarly to neural cells, it also stimulates angiogenesis by promoting elongation and migration of endothelial cells.,TNC, Hexabrachion, Cytotactin, Neuronectin

Gene ID: 3371

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development