SYK antibody (AA 5-360)

Catalog No. ABIN94475

Product Details anti-SYK Antibody

Target: SYK (Spleen tyrosine Kinase (SYK))

Binding Specificity: AA 5-360

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk, an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Cross-Reactivity (Details): Mouse, Human, Rat, Other not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant fragment (aa 5-360) of human Syk.

Clone: SYK-01

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: tonsil B cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-SYK antibody (ABIN94475)

Hasan, Rahman, Sebo, Osicka: "Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling." in: Toxins, Vol. 11, Issue 6, (2020) (PubMed).

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, (2002) (PubMed).

Arudchandran, Brown, Peirce, Song, Zhang, Siraganian, Blank, Rivera: "The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1." in: The Journal of experimental medicine, Vol. 191, Issue 1, pp. 47-60, (2000) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk (SYK Products)

Synonyms: p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody

Background: Spleen associated tyrosine kinase,Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.,p72Syk

Gene ID: 6850

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling