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Phosphotyrosine antibody

Name: Phosphotyrosine antibody
Brand: antibodies-online
SKU: ABIN94452
Price: 372.97 USD
Availability: InStock

Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/mL, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel). (Phosphotyrosine antibody)

FACS

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor ® 488 (green). (Phosphotyrosine antibody)

2 images

(3 references)

Catalog No. ABIN94452

Datasheet as PDF

Target See all Phosphotyrosine products

Phosphotyrosine
Reactivity
15

15

15

3
Various Species
Host
84

27

1
Mouse
Compatible Secondaries
Clonality
94

18
Monoclonal
Conjugate
35

17

10

8

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
Application
79

60

38

38

32

21

20

17

13

9

7

7

7

6

3

2

2

2

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Cross-Reactivity (Details)

Broad

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Phosphotyrosine conjugated to bovine serum albumin.

Clone

P-Tyr-01

Isotype

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Atto 594

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 PE

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM Host: Mouse Monoclonal PY20 unconjugated

Application Notes

Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." in: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
Target

Phosphotyrosine

Abstract

Phosphotyrosine Products

Target Type

Amino Acid

Background

PY