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HLAG antibody

HLAG Reactivity: Human WB, IHC (fro), IHC (p) Host: Mouse Monoclonal MEM-G-1 unconjugated
Catalog No. ABIN94366
  • Target See all HLAG Antibodies
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    Reactivity
    • 124
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 68
    • 61
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 73
    • 61
    Monoclonal
    Conjugate
    • 49
    • 19
    • 18
    • 14
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLAG antibody is un-conjugated
    Application
    • 80
    • 66
    • 58
    • 44
    • 28
    • 25
    • 13
    • 13
    • 11
    • 10
    • 8
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    The antibody MEM-G/1 reacts with an extracellular epitope of denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Denatured bacterially expressed recombinant human HLA-G heavy chain.
    Clone
    MEM-G-1
    Isotype
    IgG1
  • Application Notes
    Immunohistochemistry (paraffin sections): Recommended dilution: 1:60 to 1:100, incubation: 1 h at RT, positive tissue: human placenta - extravillous cytotrophoblast, heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven).
    Western blotting: Recommended dilution 1-2 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Jasinski-Bergner, Stehle, Gonschorek, Kalich, Schulz, Huettelmaier, Braun, Seliger: "Identification of 14-3-3? Gene as a Novel miR-152 Target Using a Proteome-based Approach." in: The Journal of biological chemistry, Vol. 289, Issue 45, pp. 31121-35, (2014) (PubMed).

    Amiot, Vu, Rauch, LHelgoualch, Chalmel, Gascan, Turlin, Guyader, Samson: "Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis." in: Journal of hepatology, Vol. 60, Issue 2, pp. 245-52, (2014) (PubMed).

    Larsen, Bzorek, Pass, Larsen, Nielsen, Svendsen, Lindhard, Hviid: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, (2011) (PubMed).

    Biondi, Ferretti, Lunghi, Medici, Cervellati, Pavan, Vesce, Morano, Adinolfi, Bertoni, Abelli: "cAMP efflux from human trophoblast cell lines: a role for Multidrug Resistance Protein (MRP)1 transporter." in: Molecular human reproduction, (2010) (PubMed).

    Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).

    Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, (2004) (PubMed).

    Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, (2002) (PubMed).

    Hurks, Valter, Wilson, Hilgert, van den Elsen, Jager: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, (2001) (PubMed).

    Frumento, Franchello, Palmisano, Nicotra, Giacomini, Loke, Geraghty, Maio, Manzo, Natali, Ferrara: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, (2000) (PubMed).

    Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, (2000) (PubMed).

  • Target
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    Alternative Name
    HLA-G (HLAG Products)
    Synonyms
    MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
    Background
    Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
    Gene ID
    3135
    UniProt
    P17693
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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