HLA-DQ1/DQ3 antibody

Catalog No. ABIN94338

Product Details anti-HLA-DQ1/DQ3 Antibody

Target: HLA-DQ1/DQ3 (MHC Class II HLA-DQ1/DQ3 (HLA-DQ1/DQ3))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DQ1/DQ3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity: The antibody HL-37 reacts with extracellular polymorphic determinant on human HLA-DQ1 and HLA-DQ3 Molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human, Porcine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Burkitt's lymphoma cell line Raji.

Clone: HL-37

Isotype: IgG3

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-HLA-DQ1/DQ3 antibody (ABIN94338)

Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).

Target Details for HLA-DQ1/DQ3

Target: HLA-DQ1/DQ3 (MHC Class II HLA-DQ1/DQ3 (HLA-DQ1/DQ3))

Alternative Name: HLA-DQ1+DQ3 (HLA-DQ1/DQ3 Products)

Background: The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 Molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).