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Keratin 5/8 antibody

KRT5/8 Reactivity: Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep WB, IHC (p), ICC, IP Host: Mouse Monoclonal C-50 unconjugated
Catalog No. ABIN94292
  • Target See all Keratin 5/8 (KRT5/8) products
    Keratin 5/8 (KRT5/8)
    Reactivity
    • 22
    • 15
    • 14
    • 12
    • 12
    • 12
    • 11
    • 9
    • 9
    • 3
    • 2
    • 2
    Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep
    Host
    • 22
    Mouse
    Clonality
    • 22
    Monoclonal
    Conjugate
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 5/8 antibody is un-conjugated
    Application
    • 16
    • 16
    • 13
    • 13
    • 8
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
    No Cross-Reactivity
    Chicken, Xenopus laevis
    Cross-Reactivity (Details)
    Human, Sheep, Canine (Dog), Hamster, Mouse, Rat, Bovine, Porcine
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Cytoskeleton preparation from HeLa cells.
    Clone
    C-50
    Isotype
    IgG1
  • Application Notes
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: human prostate.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

  • Target
    Keratin 5/8 (KRT5/8)
    Alternative Name
    Cytokeratin 5+8 (KRT5/8 Products)
    Background
    Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K5, K8, CK5, CK8, CYK5, CYK8, KRT5, KRT8
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