Cytokeratin 19 antibody

Catalog No. ABIN94290

Product Details anti-Cytokeratin 19 Antibody

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: MCF-7 human breast adenocarcinoma cell line

Clone: A53-B-A2

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: MCF-7, BA-17, HT-29 cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Cytokeratin 19 antibody (ABIN94290)

Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19 (KRT19 Products)

Synonyms: CK19 antibody, K19 antibody, K1CS antibody, AI663979 antibody, EndoC antibody, Krt-1.19 antibody, Krt1-19 antibody, Ka19 antibody, k19 antibody, ck19 antibody, k1cs antibody, krt9 antibody, krt15 antibody, MGC76282 antibody, GK-19 antibody, MGC83069 antibody, KRT19 antibody, keratin 19 antibody, keratin 19 L homeolog antibody, keratin, type I cytoskeletal 19 antibody, KRT19 antibody, Krt19 antibody, krt19 antibody, krt19.L antibody, LOC100344434 antibody, LOC101117946 antibody

Background: Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gene ID: 3880

UniProt: P08727