pan Keratin antibody

Catalog No. ABIN94275

Product Details anti-pan Keratin Antibody

Target: pan Keratin (panKRT)

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Cross-Reactivity (Details): Mammalian

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Clone: C-11

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-pan Keratin antibody (ABIN94275)

Haun, Devaraj, Marinelli, Lee, Weissleder: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, (2011) (PubMed).

Bianchi, Bombelli, Raimondo, Torsello, Angeloni, Ferrero, Di Stefano, Chinello, Cifola, Invernizzi, Brambilla, Magni, Pitto, Zanetti, Mocarelli, Perego: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, (2010) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin (Pan-reactive) (panKRT Products)

Synonyms: xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT