CD9 antibody

Catalog No. ABIN94244

Product Details anti-CD9 Antibody

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cytometry by Time of Flight (CyTOF)

Specificity: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Pre-B cell line NALM-6.

Clone: MEM-61

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 2-4 μg/mL, non-reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/mL, positive tissue: prostate.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD9 antibody (ABIN94244)

Lafleur, Xu, Hemler: "Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 2030-40, (2009) (PubMed).

Singh, Sugimoto, Dhawan, Harris: "Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance." in: American journal of physiology. Cell physiology, Vol. 293, Issue 5, pp. C1660-8, (2007) (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, (2001) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9 (CD9 Products)

Synonyms: BTCC-1 antibody, DRAP-27 antibody, MIC3 antibody, MRP-1 antibody, TSPAN-29 antibody, TSPAN29 antibody, Tspan29 antibody, cd9 antibody, wu:fb61g04 antibody, zgc:73383 antibody, zgc:85743 antibody, CD9 molecule antibody, CD9 antigen antibody, CD9 molecule a antibody, CD9 antibody, Cd9 antibody, cd9a antibody

Background: CD9 Molecule,CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.,MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, BA2

Gene ID: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization