The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5, and has blocking activity.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze.
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Omidvar, Wang, Brennan, Longhi, Smith, Morgan: "Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2915-23, (2006) (PubMed).
Stefanová, Horejsí, Ansotegui, Knapp, Stockinger: "GPI-anchored cell-surface molecules complexed to protein tyrosine kinases." in: Science (New York, N.Y.), Vol. 254, Issue 5034, pp. 1016-9, (1991) (PubMed).
Forsberg, Bazil, Stefanová, Schröder: "Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11." in: Immunogenetics, Vol. 30, Issue 3, pp. 188-93, (1989) (PubMed).
Stefanová, Hilgert, Kristofová, Brown, Low, Horejsí: "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol." in: Molecular immunology, Vol. 26, Issue 2, pp. 153-61, (1989) (PubMed).
CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN