CD45RB antibody

Catalog No. ABIN94140

Product Details anti-CD45RB Antibody

Target: CD45RB

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: The antibody MEM-55 recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human thymocytes and T lymphocytes.

Clone: MEM-55

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Flow cytometry: Recommended dilution: 0.5-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD45RB antibody (ABIN94140)

Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB (CD45RB Products)

Synonyms: B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody

Background: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.,PTPRCR, T200RB