CD41, CD61 antibody

Catalog No. ABIN94110

Product Details anti-CD41, CD61 Antibody

Target: CD41, CD61 (CD41,CD61)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD41, CD61 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC)

Specificity: The antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes, the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.

Cross-Reactivity (Details): Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bovine trombocytes.

Clone: IVA30

Isotype: IgG2

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD41, CD61 antibody (ABIN94110)

"Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 213-468, (1997) (PubMed).

Mateo, Perez de la Lastra, Moreno, Dusinsky, Bilka, Simon, Horovska, Llanes: "Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 363-70, (1997) (PubMed).

Mateo, Pintado, Perez de la Lastra, Dusinsky, Simon, Naessens, Llanes: "Ruminant cluster CD41/CD61." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 251-3, (1997) (PubMed).

Naessens, Howard, Hopkins: "Nomenclature and characterization of leukocyte differentiation antigens in ruminants." in: Immunology today, Vol. 18, Issue 8, pp. 365-8, (1997) (PubMed).

Simon, Dusinsky, Horovska, Bilka, Hluchy: "Immunohistochemical reactivity of anti-platelet monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 377-82, (1997) (PubMed).

Target Details for CD41, CD61

Target: CD41, CD61 (CD41,CD61)

Abstract: CD41, CD61 Products

Background: CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.,platelet GPIIb, Integrin alpha-IIb, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa