CD31 antibody (Biotin)
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- Target See all CD31 (PECAM1) Antibodies
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD31 antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificity
- The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- Immunogen
- Leukocytes of a patient suffering from LGL-type leukaemia
- Clone
- MEM-05
- Isotype
- IgG1
- Top Product
- Discover our top product PECAM1 Primary Antibody
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- Application Notes
- Flow cytometry: Recommended dilution: 2-4 μg/mL.
- Comment
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies." in: PLoS ONE, Vol. 7, Issue 12, pp. e52815, (2013) (PubMed).
: "Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 4, pp. 2364-71, (2001) (PubMed).
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Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies." in: PLoS ONE, Vol. 7, Issue 12, pp. e52815, (2013) (PubMed).
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- Target
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- CD31 (PECAM1 Products)
- Synonyms
- CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody
- Background
- Platelet and endothelial cell adhesion molecule 1,CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.,PECAM-1, EndoCAM, GPIIA, PECA1
- Gene ID
- 5175
- UniProt
- P16284
- Pathways
- Regulation of Actin Filament Polymerization
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