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TERT antibody (AA 597-611)

Verified TERT Reactivity: Human ELISA, FACS, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN940568
  • Target See all TERT Antibodies
    TERT (Telomerase Reverse Transcriptase (TERT))
    Binding Specificity
    • 16
    • 16
    • 10
    • 10
    • 7
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 597-611
    Reactivity
    • 102
    • 37
    • 20
    • 5
    • 1
    • 1
    Human
    Host
    • 106
    • 5
    Goat
    Clonality
    • 97
    • 14
    Polyclonal
    Conjugate
    • 50
    • 8
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This TERT antibody is un-conjugated
    Application
    • 66
    • 48
    • 37
    • 27
    • 17
    • 15
    • 13
    • 12
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    TERT (aa597-611)
    Sequence
    QLRELSEAEV RQHRE
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.
    Isotype
    IgG
    Top Product
    Discover our top product TERT Primary Antibody
  • Application Notes

    Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    TERT (Telomerase Reverse Transcriptase (TERT))
    Alternative Name
    TERT (TERT Products)
    Background
    TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2
    Gene ID
    7015
    NCBI Accession
    NP_937983, NP_001180305
    Pathways
    Telomere Maintenance
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