CD235ab antibody (N-Term) (PE)
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- Target See all CD235ab (CD235a + CD235b) products
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- Binding Specificity
- N-Term
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD235ab antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Synthetic peptide (Human, N-terminal)
- Clone
- HIR2
- Isotype
- IgG2b
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- Application Notes
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
- Comment
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- Alternative Name
- CD235ab (CD235a + CD235b Products)
- Background
- CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group
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