CD21 antibody

Catalog No. ABIN94032

Product Details anti-CD21 Antibody

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human, Pig, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody LT21 reacts with an extracellular epitope of CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.

Cross-Reactivity (Details): Human, Porcine, Bovine, Canine (Dog)

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: IM9 human B-lymphoblastoid cell line

Clone: LT21

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD21 antibody (ABIN94032)

Kulka, Brennan, Mc Gee: "Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas." in: PloS one, Vol. 17, Issue 9, pp. e0274261, (2022) (PubMed).

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21 (CR2 Products)

Synonyms: C3DR antibody, CD21 antibody, CR antibody, CVID7 antibody, SLEB9 antibody, Cd21 antibody, cd21 antibody, CR1 antibody, CR1-L antibody, CR2 antibody, complement C3d receptor 2 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, CR2 antibody, Cr2 antibody, CR1 antibody

Background: Complement C3d receptor 2,CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.,CR2, C3DR2, CVID7, SLEB9

Gene ID: 1380

UniProt: P20023

Pathways: Complement System