CD15 antibody

Catalog No. ABIN93984

Product Details anti-CD15 Antibody

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytometry by Time of Flight (CyTOF)

Specificity: The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells.

No Cross-Reactivity: Cow, Pig, Sheep

Cross-Reactivity (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human granulocytes

Clone: MEM-158

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 (FUT4 Products)

Synonyms: AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody

Background: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.,Lewis x Blood Group antigen, Le(x), SSEA-1, 3-fucosyl-N-acetyllactosamine