Blood Group ABH antibody

Catalog No. ABIN93921

Product Details anti-Blood Group ABH Antibody

Target: Blood Group ABH

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Blood Group ABH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe^b. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.

Cross-Reactivity (Details): Human

Purification: Hybridoma culture supernatant concentrated by membrane ultrafiltration.

Endotoxin Level: Low Endotoxin

Immunogen: Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Clone: HE-10

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry (paraffin sections): The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0.
Flow cytometry: Recommended dilution: 1-3 : 100.

Comment:

The hybridoma culture supernatant is 4 x concentrated by ultrafiltration using 100 kDa-cut off membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Blood Group ABH antibody (ABIN93921)

Vanák, Drímalová, Smyslová, N?mec, Viklický, Wisniewski: "Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities." in: Neoplasma, Vol. 36, Issue 4, pp. 479-88, (1989) (PubMed).

N?mec, Drímalová, Horejsí, Vanák, Bártek, Viklický: "Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine." in: Vox sanguinis, Vol. 52, Issue 1-2, pp. 125-8, (1987) (PubMed).

Target Details for Blood Group ABH

Target: Blood Group ABH

Abstract: Blood Group ABH Products