TUBB antibody

Catalog No. ABIN93914

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Pig, Chicken, Tetrahymena, Fish, Paramecium, Nicotiana tabacum, Arabidopsis

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.

Cross-Reactivity (Details): Broad species reactivity

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Beta-subunits of porcine brain tubulin.

Clone: TU-06

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry: Recommended dilution: 2 μg/mL, fixed and permeabilized cells, positive control: 3T3 mouse embryonal fibroblast cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: heart.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TUBB antibody (ABIN93914)

Tobita, Liu, Janczewski, Tinney, Nonemaker, Augustine, Stolz, Shroff, Keller: "Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 4, pp. H1829-37, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Janisch, Hozák, Dráber: "Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex." in: Protoplasma, Vol. 225, Issue 3-4, pp. 157-67, (2005) (PubMed).

Solecki, Model, Gaetz, Kapoor, Hatten: "Par6alpha signaling controls glial-guided neuronal migration." in: Nature neuroscience, Vol. 7, Issue 11, pp. 1195-203, (2004) (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-tubulin (TUBB Products)

Synonyms: M40 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, XLOT antibody, m40 antibody, ok/sw-cl.56 antibody, tubb1 antibody, tubb5 antibody, TUBB2A antibody, TUBB2B antibody, TUBB antibody, 1t antibody, B1t antibody, BETA 56D antibody, CG9277 antibody, DTB2 antibody, Dmbeta1 antibody, Dmel\\CG9277 antibody, T antibody, Tub antibody, Tubulin antibody, beta antibody, beta-Tub antibody, beta-Tub56D antibody, beta-tub antibody, beta-tubulin56D antibody, beta1 antibody, beta1-Tubulin antibody, beta1-tub antibody, beta1Tub antibody, beta1t antibody, beta1tub antibody, beta56D antibody, betaTub antibody, betaTub1 antibody, beta[[1]] tubulin antibody, beta[[1]]-tubulin antibody, betatub(56D) antibody, 143391_i_at antibody, 3t antibody, B3t antibody, BETA 60D antibody, CG3401 antibody, D.m.BETA-60D antibody, DTB3 antibody, Dmbeta3 antibody, Dmel\\CG3401 antibody, Tub60D antibody, beta-Tub60D antibody, beta-Tub6D antibody, beta3 antibody, beta3 TU antibody, beta3-Tub antibody, beta3-tubulin antibody, beta3TUB antibody, beta3Tub antibody, beta3t antibody, beta60C antibody, betaTub3 antibody, betaTub60C antibody, beta[[3]] tubulin antibody, beta[[3]]-Tub antibody, beta[[3]]-tubulin antibody, betatub60D antibody, p50 antibody, p50/tubulin antibody, p53 antibody, p53/tubulin antibody, 2t antibody, B2t antibody, BETA 85D antibody, BETA2 antibody, CG9359 antibody, D.m.BETA-85D antibody, DTB4 antibody, Dmbeta2 antibody, Dmel\\CG9359 antibody, beta(2)Tu antibody, beta(2)Tub antibody, beta-Tub85D antibody, beta-tub85D antibody, beta2 antibody, beta2-tub antibody, beta2t antibody, beta2tub antibody, beta85D antibody, betaTub2 antibody, beta[[2]]-tubulin antibody, ms(3)KK[D] antibody, 4t antibody, B4t antibody, BETA 98B antibody, CG4869 antibody, DTB1 antibody, Dmbeta4 antibody, Dmel\\CG4869 antibody, beta-Tub97EF antibody, beta4 antibody, beta4t antibody, beta97F antibody, betaTub4 antibody, betaTub98BC antibody, betaTub98C antibody, Tub1 antibody, bmtub1 antibody, Tub3 antibody, bmtub3 antibody, Tub2 antibody, bmtub2 antibody, Tub4 antibody, bmtub4 antibody, LOC100101153 antibody, tubulin beta class I antibody, putative cobalt-zinc-cadmium outer membrane resistance protein antibody, Tubulin beta chain antibody, tubulin beta-2A chain antibody, beta-Tubulin at 56D antibody, beta-Tubulin at 60D antibody, beta-Tubulin at 85D antibody, beta-Tubulin at 97EF antibody, beta-tubulin antibody, tubulin beta 6 class V antibody, beta tubulin antibody, tubulin beta class I L homeolog antibody, tubulin, beta 5 class I antibody, TUBB antibody, czcC antibody, tbb-6 antibody, tubb antibody, LOC462399 antibody, betaTub56D antibody, betaTub60D antibody, betaTub85D antibody, betaTub97EF antibody, Tub1 antibody, Tub3 antibody, Tub2 antibody, Tub4 antibody, TVAG_525430 antibody, TVAG_062880 antibody, TVAG_456920 antibody, LOC100101153 antibody, TUBB6 antibody, Tb927.1.2330 antibody, Tb927.1.2350 antibody, Tb927.1.2370 antibody, Tb927.1.2390 antibody, tubb.L antibody, Tubb antibody, LOC693049 antibody, LOC443015 antibody, Tubb5 antibody

Background: Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB

Gene ID: 81027

UniProt: Q9H4B7

Pathways: Microtubule Dynamics, M Phase