TUBB3 antibody (N-Term)

Catalog No. ABIN93911

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Cross-Reactivity (Details): Broad species reactivity

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Clone: TU-20

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL, positive control: porcine brain lysate, negative control: HPB-ALL human peripheral blood leukemia cell line, reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, standard ABC technique (DAB+), pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or high temperature citrate buffer antigen retrieval, positive tissue: neuronal tissue.
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TUBB3 antibody (ABIN93911)

Letellier, Szíber, Chamma, Saphy, Papasideri, Tessier, Sainlos, Czöndör, Thoumine: "A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation." in: Nature communications, Vol. 9, Issue 1, pp. 3979, (2019) (PubMed).

Gudernova, Balek, Varecha, Kucerova, Kunova Bosakova, Fafilek, Palusova, Uldrijan, Trantirek, Krejci: "Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480." in: Oncotarget, Vol. 8, Issue 65, pp. 109319-109331, (2017) (PubMed).

Szíber, Liliom, Morales, Ignácz, Rátkai, Ellwanger, Link, Szűcs, Hausser, Schlett: "Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover." in: Molecular biology of the cell, Vol. 28, Issue 2, pp. 285-295, (2017) (PubMed).

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, (2011) (PubMed).

Kunova, Matulka, Eiselleova, Trckova, Hampl, Dvorak: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, (2010) (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: betaIII-tubulin (TUBB3 Products)

Background: Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3

Gene ID: 10381

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase