beta-2 Microglobulin antibody (Biotin)
(4 references)-
- Target See all beta-2 Microglobulin (B2M) Antibodies
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This beta-2 Microglobulin antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M, extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
- No Cross-Reactivity
- Chicken, Cow, Dog, Mouse, Rabbit
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- Immunogen
- Purified human beta2-microglobulin
- Clone
- B2M-01
- Isotype
- IgG2a
- Top Product
- Discover our top product B2M Primary Antibody
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anti-beta-2-Microglobulin (B2M) (AA 21-100) antibody
B2M Reactivity: Human WB, FACS, ELISA, IHC, ICC Host: Mouse Monoclonal 4G5A1 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 21-100) antibodyB2M Reactivity: Human WB, FACS, ELISA, IHC Host: Mouse Monoclonal 3G5H8 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 1-119) antibodyB2M Reactivity: Human WB, ELISA, IF, IHC (p), RNAi, PLA Host: Mouse Monoclonal 3F9-2C2 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal A2 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human WB, FACS, ELISA, IP, IHC (p), ICC, RIA Host: Mouse Monoclonal B2M-01 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate FACS, IHC, IF, StM Host: Mouse Monoclonal rB2M-961 unconjugated Recombinant Antibody
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate IHC, StM Host: Mouse Monoclonal B2M-1118 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate WB, FACS, IHC, IF, StM Host: Mouse Monoclonal B2M-961 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate FACS, IF, IHC (p) Host: Mouse Monoclonal B2M-961 unconjugated
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- Application Notes
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Flow cytometry: Recommended dilution: 1-2 μg/mL, positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions. - Comment
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).
: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, (2006) (PubMed).
: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, (2005) (PubMed).
: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).
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: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).
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- Target
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- Alternative Name
- beta2-Microglobulin (B2M Products)
- Synonyms
- Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
- Background
- Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M
- Gene ID
- 567
- UniProt
- P61769
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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