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alpha Fetoprotein antibody

AFP Reactivity: Human WB, ELISA, IP, ICC Host: Mouse Monoclonal AFP-01 unconjugated
Catalog No. ABIN93889
  • Target See all alpha Fetoprotein (AFP) Antibodies
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    Reactivity
    • 245
    • 52
    • 47
    • 28
    • 23
    • 15
    • 4
    • 4
    • 4
    • 2
    • 1
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    Human
    Host
    • 147
    • 141
    • 8
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    Mouse
    Clonality
    • 158
    • 140
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    Monoclonal
    Conjugate
    • 140
    • 39
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    • 9
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This alpha Fetoprotein antibody is un-conjugated
    Application
    • 152
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
    Cross-Reactivity (Details)
    Other not tested, Human
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    purified alpha-Fetoprotein
    Clone
    AFP-01
    Isotype
    IgG1
    Top Product
    Discover our top product AFP Primary Antibody
  • Application Notes
    ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
    Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.
    Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

  • Target
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    Alternative Name
    alpha-Fetoprotein (AFP Products)
    Synonyms
    FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
    Background
    Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP
    Gene ID
    174
    UniProt
    P02771
    Pathways
    C21-Steroid Hormone Metabolic Process
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