BrdU antibody

Catalog No. ABIN93883

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: 5-bromodeoxyuridine conjugated with hemocyanine.

Clone: MoBu-1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-2 μg/mL. Intracellular staining.
Immunocytochemistry: Recommended dilution: 2 μg/mL.
Immunohistochemistry (paraffin sections): Excellent.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-BrdU antibody (ABIN93883)

Sati, Bonev, Szabo, Jost, Bensadoun, Serra, Loubiere, Papadopoulos, Rivera-Mulia, Fritsch, Bouret, Castillo, Gelpi, Orozco, Vaillant, Pellestor, Bantignies, Marti-Renom, Gilbert, Lemaitre, Cavalli: "4D Genome Rewiring during Oncogene-Induced and Replicative Senescence." in: Molecular cell, Vol. 78, Issue 3, pp. 522-538.e9, (2020) (PubMed).

Schoonen, Kok, Wierenga, Bakker, Foijer, Spierings, van Vugt: "Premature mitotic entry induced by ATR inhibition potentiates olaparib inhibition-mediated genomic instability, inflammatory signaling, and cytotoxicity in BRCA2-deficient cancer cells." in: Molecular oncology, Vol. 13, Issue 11, pp. 2422-2440, (2020) (PubMed).

Babic, Ondrejcakova, Bakos, Racekova, Jezova: "Cell proliferation in the hippocampus and in the heart is modified by exposure to repeated stress and treatment with memantine." in: Journal of psychiatric research, Vol. 46, Issue 4, pp. 526-32, (2012) (PubMed).

Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." in: European journal of cell biology, Vol. 79, Issue 3, pp. 202-7, (2000) (PubMed).

Buckiová, Kubínová, Soukup, Jelínek, Brown: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." in: The International journal of developmental biology, Vol. 42, Issue 5, pp. 737-40, (1998) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: 5-bromodeoxyuridine (BrdU) (BrdU Products)

Target Type: Chemical

Background: Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.,5-bromodeoxyuridine