LHCB5 antibody

Catalog No. ABIN93515

Product Details anti-LHCB5 Antibody

Target: LHCB5 (Chlorophyll A-B Binding Protein CP26 (LHCB5))

Reactivity: Zea mays, Arabidopsis thaliana, Barley, Oryza sativa, Pisum sativum, Spinach

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity (Details): Peptide target used to elicit this antibody has a weak, sporadic conservation withGlutamine Synthetase to III, antibody not expected to detect this enzyme. Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase)(GLNRS), but antibody not expected to detect this enzyme.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 26 / 25-26 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogen: BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein UniProt: Q9XF89, TAIR: At4g10340 sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 1000, detected with standard ECL (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-LHCB5 antibody (ABIN93515)

Zienkiewicz, Ferenc, Wasilewska, Romanowska: "High light stimulates Deg1-dependent cleavage of the minor LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana." in: Planta, Vol. 235, Issue 2, pp. 279-88, (2012) (PubMed).

Jung, Lee, Lee, Kang, Kim, Grimm, Back: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, (2008) (PubMed).

Target Details for LHCB5

Target: LHCB5 (Chlorophyll A-B Binding Protein CP26 (LHCB5))

Alternative Name: CP26 chlorophyll a/b-binding protein of plant PSII (Lhcb5)

Synonyms: CHLOROPHYLL PROTEIN 26 antibody, CP 26 antibody, CP26 antibody, F24G24.140 antibody, F24G24_140 antibody, light harvesting complex of photosystem II 5 antibody, light harvesting complex of photosystem II 5 antibody, LHCB5 antibody

Background: AGI Code: At4g10340
Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.

Molecular Weight: expected: 26 kDa, apparent: 25-26 kDa for Arabidopsis thaliana

UniProt: Q9XF89