Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Lhcb2 antibody

Lhcb2 Reactivity: Nicotiana tabacum, Oryza sativa, Pisum sativum, Arabidopsis thaliana, Spinach, Barley, Zea mays, Chlamydomonas reinhardtii, Green bean, Moss IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN93509
  • Target
    Lhcb2 (Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2))
    Reactivity
    Nicotiana tabacum, Oryza sativa, Pisum sativum, Arabidopsis thaliana, Spinach, Barley, Zea mays, Chlamydomonas reinhardtii, Green bean, Moss
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
    Purification
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: At2g05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:At2g05070, Lhcb2.3 UniProt:Q9XF87, TAIR:At2g27690
    Isotype
    IgG
  • Application Notes
    Recommended Dilution: 1 : 5000 with standard ECL (WB).
    Comment

    Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
  • Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).

    Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

    Yuan, Xu, Yuan, Chen, Du, Xu, Zhang, Guo, Zhao, Lin: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, (2010) (PubMed).

    Tikkanen, Nurmi, Kangasjaervi, Aro: "Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light." in: Biochimica et biophysica acta, (2008) (PubMed).

    Tikkanen, Nurmi, Suorsa, Danielsson, Mamedov, Styring, Aro: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, (2008) (PubMed).

  • Target
    Lhcb2 (Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2))
    Background
    AGI Code: At2g05100
    The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476.
    Molecular Weight
    expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
    UniProt
    Q9XF86, Q9XF87, Q9SHR7, Q9S7J7
You are here:
Support