KLH antibody
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- Target See all KLH Antibodies
- KLH (Keyhole Limpet Hemocyanin (KLH))
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Reactivity
- Keyhole Limpet
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KLH antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunolocalization (IL)
- Predicted Reactivity
- Megathura crenulata - most commonly used carrier protein
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: Aprox. 400 kDa
- Purification
- serum
- Immunogen
- purified keyhole limpet hemocyanin (KLH), whole molecule
- Isotype
- IgG
- Top Product
- Discover our top product KLH Primary Antibody
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH Reactivity: Keyhole Limpet ELISA, WB Host: Rabbit Polyclonal unconjugated
anti-Keyhole Limpet Hemocyanin (KLH) antibody (FITC)KLH Reactivity: Keyhole Limpet ELISA, WB, FM Host: Rabbit Polyclonal FITC
anti-Keyhole Limpet Hemocyanin (KLH) antibody (HRP)KLH Reactivity: Keyhole Limpet ELISA, WB Host: Rabbit Polyclonal HRP
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Rhodamine)KLH Reactivity: Keyhole Limpet ELISA, WB, FM Host: Rabbit Polyclonal Rhodamine
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Alkaline Phosphatase (AP))KLH Reactivity: Keyhole Limpet ELISA Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Biotin)KLH Reactivity: Keyhole Limpet ELISA, WB Host: Rabbit Polyclonal Biotin
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Texas Red (TR))KLH Reactivity: Keyhole Limpet ELISA, WB, FM Host: Rabbit Polyclonal Texas Red (TR)
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- Application Notes
- Recommended Dilution: 1 : 10 000 (ELISA), 1 : 10 000 (WB), 1 : 1000 (IL).
- Comment
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Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution please add 50 µL of dest. water
- Buffer
- PBS pH 7.4
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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- Target
- KLH (Keyhole Limpet Hemocyanin (KLH))
- Abstract
- KLH Products
- Background
- Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
- Molecular Weight
- ca. 400 kDa/subunit
- UniProt
- Q6KC56
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