HIV1RT antibody (Biotin)
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- Target See all HIV1RT products
- HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))
- Reactivity
- Human Immunodeficiency Virus (HIV)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HIV1RT antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- This antibody binds to recombinant rev (HIV1, HxB2, HxB3) in ELISA and Western Blot
- Purification
- purified
- Purity
- > 95 % pure
- Immunogen
- HIV1 Rev antibody was purified in mouse using recombinant Rev (HIV-1, HxB2, HxB3) as the immunogen.
- Clone
- 9G2G4D6E8
- Isotype
- IgG2b
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- Application Notes
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ELISA: 1:500-1:1000, WB: 1:500-1:1000
Optimal conditions should be determined by the investigator. - Comment
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Binds to recombinant rev (HIV-1, HxB2, HxB3) in ELISA and Western Blot
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile distilled water.
- Concentration
- Lot specific
- Buffer
- Supplied lyophilized as a purified IgG in PBS buffer with no preservatives added
- Preservative
- Without preservative
- Precaution of Use
- Not tested for sterility or endotoxin content.
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-80 °C
- Storage Comment
- Store lyophilized product at 2-8 °C short term, following reconstitution, aliquot and store at -70 °C or lower.
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- Target
- HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))
- Alternative Name
- HIV1 REV (HIV1RT Products)
- Target Type
- Viral Protein
- Background
- Rev is a HIV gene. The name rev stands for Regulator of Virion. The gene's protein product allows fragments of HIV mRNA that contain a Rev Response Element (RRE) to be exported from the nucleus to the cytoplasm. In the absence of the rev gene, the host RNA splicing machinery in the nucleus quickly splices the RNA so that only the smaller, regulatory proteins can be produced, in the presence of rev, RNA is exported from the nucleus before it can be spliced, so that the structural proteins and RNA genome can be produced. Synonyms: Monoclonal HIV1 Rev antibody, Anti-HIV1 Rev antibody, Anti-repression transactivator protein antibody, art/trs antibody, HIV1gp6 antibody, Human immunodeficiency virus 1 Rev antibody, Protein Rev antibody, Regulator of expression of viral proteins antibody, Rev antibody.
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