V5 Epitope Tag antibody

Catalog No. ABIN933572

Product Details anti-V5 Epitope Tag Antibody

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Sequence: GKPIPNPLLG LDST

Purification: Protein A affinity chromatography

Immunogen: V5 Tag antibody was raised in mouse using GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH as the immunogen.

Clone: E10

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5 Tag (V5 Epitope Tag Products)

Target Type: Tag

Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This tag is particularly useful for western blotting and immunoprecipitation experiments and may also find use in antibody purification.    . Synonyms: Monoclonal V5 Tag antibody, anti-V5 Tag antibody, E10 antibody, V4RR antibody, V5 antibody.