RFP antibody (N-Term)
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- Target See all RFP Antibodies
- RFP (Red Fluorescent Protein (RFP))
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Binding Specificity
- N-Term
- Reactivity
- Discosoma
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This RFP antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)
- Purification
- Protein A affinity chromatography
- Immunogen
- RFP Tag antibody was raised in mouse using RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates as the immunogen.
- Clone
- RF5R
- Isotype
- IgG1
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- Application Notes
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WB: 1:1000-3000
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Abstract
- RFP Products
- Background
- Red Fluorescent Protein is a protein which exhibits bright red fluorescence when exposed to blue light. Fluorescent proteins are members of a structurally homologous class of proteins that share the unique property of being self-sufficient to form a visible wavelength chromophore from a sequence of 3 amino acids within their own polypeptide sequence. It is common research practice for biologists to introduce a gene encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy. Synonyms: Monoclonal RFP Tag antibody, anti-RFP Tag antibody, RFP antibody, Red Fluorescent Protein antibody, RF5R antibody.
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