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GFP antibody (N-Term)

Name: GFP antibody (N-Term)
Brand: antibodies-online
SKU: ABIN933569
Price: 508.55 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IP, DB Host: Mouse Monoclonal GF28R unconjugated

1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. (GFP antibody (N-Term))

1 image

Catalog No. ABIN933569

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Binding Specificity
16

15

10

8

4

3

3

3

2

2

2

1

1

1

1

1

1

1
N-Term
Reactivity
175

15

11

9
Aequorea victoria
Host
63

62

35

15

9

5

1

1
Mouse
Compatible Secondaries
Clonality
115

71
Monoclonal
Conjugate
81

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
149

75

46

33

25

25

24

21

14

13

13

12

12

5

4

4

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Purification

Protein A affinity chromatography

Immunogen

GFP antibody was raised in mouse using GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates as the immunogen.

Clone

GF28R

Isotype

IgG1

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Application Notes

WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative

Sodium azide

Precaution of Use

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. Synonyms: Monoclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody, GFP tag antibody.