EIF2S1 antibody

Catalog No. ABIN933102

Product Details anti-EIF2S1 Antibody

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Dot Blot (DB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: EIF2 S1 antibody was raised in mouse using recombinant Human Eukaryotic Translation Initiation Factor 2, Subunit 1 Alpha, 32 da

Clone: EIF2S1A2B8

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: EIF2 S1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1 (EIF2S1 Products)

Background: The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kDa), beta (38 kDa, MIM 603908), and gamma (52 kDa, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha. Synonyms: Monoclonal EIF2S1 antibody, Anti-EIF2S1 antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, EIF-2A antibody, EIF-2alpha antibody, EIF-2 antibody, EIF2A antibody.

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C