NDC80 antibody

Catalog No. ABIN931089

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB)

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: KNTC2 antibody was raised in mouse using recombinant Human Ndc80 Homolog, Kinetochore Complex Component (S.Cerevisiae) (Ndc80)

Clone: 2497C3a

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: KNTC2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: KNTC2 (NDC80 Products)

Synonyms: CG9938 antibody, Dmel\\CG9938 antibody, Hec1 antibody, NDC80 antibody, dNdc80 antibody, dmNdc80 antibody, hec1 antibody, KNTC2 antibody, ndc10 antibody, tid3 antibody, 2610020P18Rik antibody, AV139730 antibody, HEC antibody, HEC1 antibody, Kntc2 antibody, kntc2l antibody, wu:fb52e02 antibody, zgc:111801 antibody, HsHec1 antibody, TID3 antibody, hsNDC80 antibody, kntc2 antibody, NDC80, kinetochore complex component antibody, CG9938 gene product from transcript CG9938-RA antibody, spindle pole body protein Ndc80 antibody, kinetochore protein Ndc80 antibody, NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, NDC80 kinetochore complex component antibody, NDC80 kinetochore complex component S homeolog antibody, NDC80 antibody, Ndc80 antibody, ndc80 antibody, ndc80.S antibody

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved. Synonyms: Monoclonal KNTC2 antibody, Anti-KNTC2 antibody, Kinetochore associated 2 antibody, NDC80 antibody, HEC antibody, HEC1 antibody, TID3 antibody, hsNDC80 antibody.

Pathways: Maintenance of Protein Location