Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
Cross-Reactivity
Fish, Cow (Bovine), Mouse (Murine), Rat (Rattus), Trout, Kangaroo
Purification
Protein A affinity chromatography
Immunogen
Lamin antibody was raised in mouse using Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells) as the immunogen.
Clone
X67
Isotype
IgG1
Application Notes
IHC: 1:10 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in distilled water. Final solution contains contains 0.09 % NaN3
Concentration
Lot specific
Buffer
Supplied in lyophilized form.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 2-8 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Target
Lamin
Background
Nuclear lamins, also known as Class V intermediate filaments, are fibrous proteins providing structural function and transcriptional regulation in the cell nucleus. Nuclear lamins interact with membrane-associated proteins to form the nuclear lamina on the interior of the nuclear envelope. They are involved in the breakdown and reformation of the nuclear envelope during mitosis, as well as the positioning of nuclear pores. Synonyms: Monoclonal Lamin antibody, Anti Lamin antibody.