Actin antibody (N-Term)

Catalog No. ABIN930825

Product Details anti-Actin Antibody

Target: Actin

Binding Specificity: N-Term

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Cow (Bovine), Horse (Equine), Rat (Rattus), Mouse (Murine), Chicken

Purification: Protein A affinity chromatography

Immunogen: Actin antibody was raised in mouse using synthetic N- terminus decapeptide of alpha-smooth-muscle isoform of actin as the immunogen.

Clone: ASM-1

Isotype: IgG2a

Application Details

Application Notes: Optimal conditions should be determined byt he investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied in liquid form, contains 0.09 % NaN3 and 0.5 % BSA in PBS pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Actin

Target: Actin

Abstract: Actin Products

Background: Actin is a globular, roughly 42-kDa moonlighting protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Synonyms: Monoclonal Smooth Muscle Actin antibody, Anti Smooth Muscle Actin antibody.