FZR1 antibody (N-Term)

Catalog No. ABIN928538

Product Details anti-FZR1 Antibody

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine)

Purification: Purified

Immunogen: Fzr1 antibody was raised in rabbit using the N terminal of Fzr1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Fzr1 Blocking Peptide, catalog no. 33R-8121, is also available for use as a blocking control in assays to test for specificity of this Fzr1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: Fzr1 (FZR1 Products)

Synonyms: CDH1-C antibody, FYR antibody, FZR antibody, CDH1 antibody, fzr1 antibody, CDC20C antibody, FZR2 antibody, HCDH antibody, HCDH1 antibody, AW108046 antibody, Cdh1 antibody, Fyr antibody, zgc:55450 antibody, fizzy and cell division cycle 20 related 1 antibody, fizzy/cell division cycle 20 related 1 antibody, fizzy-related protein homolog antibody, fizzy/cell division cycle 20 related 1 (Drosophila) antibody, fizzy/cell division cycle 20 related 1a antibody, Fzr1 antibody, CDH1-C antibody, FZR1 antibody, LOC100027495 antibody, LOC100179951 antibody, fzr1a antibody

Background: Fzr1 is the key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Fzr1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Synonyms: Polyclonal Fzr1 antibody, Anti-Fzr1 antibody, fizzy/cell division cycle 20 related 1, Drosophila antibody, AW108046 antibody, Cdh1 antibody, FZR antibody, FZR2 antibody, Fyr antibody, HCDH antibody, HCDH1 antibody.

Pathways: DNA Replication, Synthesis of DNA